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Colour Waves on Squids -
Andrew's Squid Experiments



About

Orderly colour changes of cephalopods normally require an intact brain. When the brain has died, or the nerve supply to the skin has been removed, the colours become chaotic. Such autonomous behaviour highlights a general principle.

Andrew Packard [AP95], [AP01], [AP06], [AP11], [APgl] has observed wave-like excitements flowing through the coloured spots (chromatophore organs) on one side of the mantle of these animals some days after cutting its nerve supply from the brain to one side of the body. These muscle-operated organs develop supersensitivity and communication between them. The different properties can be inspected directly through the animal's translucent skin.

The idea of slow flowing information in communication systems of every kind, including the nervous system, was suggested in the 1993 book by Heinz "Neuronale Interferenzen" [NI93]. Andrews pictures and films show, that bio-systems are able to form discrete "waves", that means waves on wires, in all bio-systems.

Interesting physical "wave interference" properties, like a geometrical wave length, can be observed with our eyes.


Observations

Waves proceeding in a layered 'neural' wave space show relations within a single layer and between different layers. Black of different size class, and yellow chromatophores act relatively independently of each other, and with different conductivities in the separate layers (Fig.13).

Beside straight-going waves proceeding from boundaries with innervated skin, we find circular waves radiating from a centre of irritation and spiral waves rotating around a stable core.

Water temperature influences the waves. Below 6°C they are abolished. Above 10°C, frequency, velocity and numbers of waves differentially increase.

Deletion of two colliding waves shows, that 1) the conductivity mechanism acts multidirectionally; 2) disappearance of waves behind the points of interference means that the wave space is cleaned during a chromatophore's refractory period.




Fig.0: Centimeter scale at the squid


Movies

Records from May 31, 1995



Fig.1: A black colour wave moves along the denervated side of a squid




Fig.2: Pressure induced waves moving from left (head) to right




Fig.3: Circular waves coming from center region



Records from October 27, 1995



Fig.4: Straight going waves from left (head) to right




Fig.5: Circular waves coming from center region




Fig.6: Anti-clockwise turning spiral waves




Fig.7: Clockwise turning spiral waves




Fig.8: Slower wave movement induced by cold water (8°C)




Fig.9: Externally stimulated circular wave




Fig.10: Yellow and black colour waves moving independently with only weak influence on each other




Fig.11: Two waves delete by collision




Fig.12: Two waves form a new wavefront by vector addition


Record from November, 1998



Fig.13: Details of Chromatophore array (screen size ~ 2x3 cm) during passage of dark and yellow waves by opening and closure of several size classes.









Fig.14: Picture series of the running black colour wave of Fig.1


Andrews experiments verify important parts of the theory about interference networks, published in the book "Neuronale Interferenzen" [NI93], in a simple, visible way. So these experiments are most interesting for students, who try to understand the world of information processing in biological systems.



Discussion

From an anatomical view, Andrew Packard discussed the results in detail in his papers [AP95], [AP01], [AP06] and [AP11]. In short form, in physical terms the squid experiments demonstrate following effects:

  1. Between muscle-activated chromatophore cells any excitement moves wave-like from cell to cell
  2. Velocity, frequency and number of waves is influenced by temperature (Fig.8)
  3. Waves of different colour (yellow and black) seem to have independent information exchange systems (nets), waves do not influence waves of different color (Fig.10)
  4. Between the chromatophores of one colour a bi-directional information exchange to the neighbours in all directions (Fig.1...12) can be observed, indicated by a wave movement in all directions
  5. Waves end normally at the end of the field by unknown inhibition or damping effects (Fig.9)
  6. Wave velocity varies direction-dependantly, indicated by spiral waves, (Fig.6).
  7. Fig.1, Fig.11 and Fig.12 show a velocity v of the wave movement of approximately v = 5cm / 3 sec = 1.6 cm/sec
  8. Using a ruler, we find in Fig.1 a geometric wave length s of approx. s = 5 mm
  9. With a velocity v of approx. 1.6 cm/sec the excitement T per cell can be found to T = s / v = 0.5 cm / (1.6 cm/sec) ≈ 0,3 s
  10. In difference to water waves, squid waves delete one another by (nonlinear) wave overlay (Fig.11, Fig.12) on the location and in the minute of touch. The effect is well-known from frog sciatica-nerve experiments
  11. For waves any destination is reachable only, if NO counter wave deletes the wave beforehand (see Fig.11, Fig.12)


Additional Discussion by Gerd Heinz:

Specific Relevance for Neuro Science

Deleting waves in Fig.11 show, that we observe a kind of inhibition (signal/wave deletion) in an excitatory network. Although wave deletion appears as a kind of inhibition here, it has nothing to do with inhibiting synapses!

A one-dimensional analogy of this experiment is the effect of contra-directional stimuli in sciatic nerve of frogs. Counter waves delete themselves because periods of refractoriness (ionic depletion zones) run behind every pulse excitement and remove any counter wave. The effect was theoretically proposed as "dynamic inhibition" in [NI93], Kap.6, S.145 and later discussed at [Bio96] or [Hz04], p.5, Fig.7b.

In addition to traditional knowledge, inhibition of signals can have different other reasons:

  1. Inhibiting substances (weights), compare to neural network theory
  2. Mask problems (delay vectors do not match), including neighbourhood inhibition
  3. Counter wave deletion (as to see in Fig.11)

Note, that No. 2) and 3) do not need any inhibiting substance or synapse.

Andrews Experiments offer a rule for the understanding of excitatory and inhibitory data flows. Waves from one source can only reach a destination, if they have a frequency, as the counter wave source. If the counter waves have higher frequency, no possibility exists, to propagate any wave to a destination against the counter waves.

Up to now it has been difficult to perceive the relevance of this effect for the understanding of nervous systems. If we suppose that the same effect (observed in this special system) happens between any kind of "normal" (bi-directional) nerve cells, one of many possible interpretations would be, that there can be no information flow on any pathway if it is blocked by counter waves. The waves with the higher frequency kills the counter wave, before the counter waves can propagate.

If we implement the idea formaly to nerve system, we can establish some laws:

Thus, to get the best chance to bring any information (as image or sign) through a nerve system, we need the highest fire frequency (before cross-interference overflow) for a certain time. This is known as a fire-burst.

The effect can be computed in relation between geometrical impulse length, refractoriness, fire frequency and empty field size, if we compute local time functions for each cell.

For computer-simulations, wave deletion encreases the time of calculations dramatically. To calculate the effect adequate, we have to calculate each cell for each time step, overlay methods for linear superimposition does not help.

Thinking this way, nerve network theory get a different direction. Although we have an excitement-only-network (without inhibition), we only have to avoid (inhibiting) wave-blocking mechanisms to get a free runway for any (multi-channel) signal between locations of source and destination. It might be possible that a certain type of special synchrony within a whole network is a possible solution, compare with Singer [SW93].

Interpretations of the effect of wave deletion reach from orthopaedics to acupuncture and kinesiology. In this fields amazing numbers of experiments are known where any sensitivity or excitement blocks a different sensitivity or excitement. The facit here: Silence is a good environment to be creative and powerful (in absence of deleting counter waves).

But there are further outstanding possibilities. Eccles [Ecc00], p. 254 remembered an experiment of Adrian and Matthews [AM34]. Using electro-encephalography they found, that any alpha-rythm of the brain (high amplitudes) can be suppressed completely by the opening of eyes. If we suppose, the frontal pulse waves input is higher with opened eyes, this higher rate suppresses probably the alpha-rythm of the brain by wave deletion effects. In other words: Is the average frequency reduced, the alpha-rythm allows, that informations reach cortical regions, that are much weaker or much further away - we seem to know this effect as "dream". Creative men also know, that we need silence to formulate a great, new idea - to reach farest regions of the brain.

In pain research, Mense [MS95] experimented with pain of rats. He found growing areas of medula spinalis produced by learned pain excitments. We know, that it is impossible to give commands for example to a broken leg. At one hand, pain produces cross-interference overflow, [NI93], Kap.5, S.104, see a simulation of the effect in [Hz98], producing a non-locality of pain. At the other hand, the high frequency of pain waves deletes counter waves, prohibiting the sending of any information back to the painful organ (Fig.11).


Acknowledgements

Thanks to Andrew Packard for inspiring discussions in the specific field.

Gerd Heinz

Sources

[AM34] Adrian, E.D. and Matthews, B.H.C. Brain 57, pp.355, 1934

[APgl] https://en.wikipedia.org/wiki/Packard_Glacier, Andrew-Packard-Glacier in Antarctica

[AP95] Packard, A.: Organization of cephalopod chromatophore systems: a neuromuscular image-generator. In: Abbott, N.J., Williamson, R., Maddock, L., Cephalopod Neurobiology, Oxford University Press, 1995, pp.331-367

[AP01] Packard, A.: 'neural' net that can be seen with the naked eye In : Backhaus. W. (ed) 2001 International School of Biocybernetics (Ischia): Neuronal coding of perceptual systems: World Scientific, Singapore, New Jersey, London, Hong Kong. pp.397-402

[AP06] Packard, A.: Contribution to the whole (H). Can squids show us anything that we did not know already? Springer, Biology and Philosophy (2006) 21, pp.189-211

[AP11] Packard, A.: Squids old and young: Scale-free design for a simple billboard. Elsevier, Optics & Laser Techn., 43 (2011), pp.302-309

[Bio96] Heinz, G., Höfs, S., Busch, C., Zöllner, M.: Time Pattern, Data Addressing, Coding, Projections and Topographic Maps between Multiple Connected Neural Fields - a Physical Approach to Neural Superimposition and Interference. http://www.gheinz.de/index.htmls BioNet'96, GFaI-Berlin, 1997, ISBN 3-00-001107-2, pp.45-57

[Ecc00] Eccles, J.C.: Das Gehirn des Menschen. Seehamer Verlag 2000

[Hz96] Heinz, G.: Physikalisch orientierte Modelle von Nervennetzen - Hypothetische Modelle und Beispiele. Document only on the web, 1996

[Hz98] Heinz, G.: Simulierter Schmerz als Überflutung aufgrund von Überfeuerung - Variation des Pulsabsstands einer dreikanaligen Pulsprojektion. Document only on the web, Sept.17, 1998

[Hz01] Heinz, G.: An investigation of 'Pictures of Thought' - properties of pulsating, short circuit networks in Theory and simulation. Int. School of Biophysics "Neuronal Coding of Perceptual Systems", Cassamicciola, Isle of Ischia , Naples, Italy, Oct.12-17, 1998. Published in Backhaus, W.: Neuronal Coding of Perceptual Systems. Series on biophysics and biocybernetics, vol.9 - Biophysics, World Scientific, New Yersey, London, Singapore, Hong Kong, 2001, ISBN 981-02-4164-X, p.377-391

[Hz04] Heinz, G.: Interference Networks - a Physical, Structural and Behavioural Approach to Nerve System. Lecture hold at "Brain Inspired Cognitive Systems" (BICS), 29 Aug. - 1 Sept. 2004, University of Stirling, Scotland, UK, paper on conference CD as #1115.pdf.
The rule played by refractoriness: Compare with page 5, Fig.7b

[MS95] Mense, S.: Neuroplastizität und chronischer Schmerz. Uni Heidelberg, Publikationen, Ruperto Carola, Ausgabe 1/95, source

[NI93] Heinz, G.: Neuronale Interferenzen (Pulsinterferenzen in Netzwerken mit verteilten Parametern). Autor gleich Herausgeber. GFaI Berlin, 1992, 1993, 1994, 1996, Persönlicher Verteiler in ca. 30 Exemplaren. 150...300 S.

[SW93] Singer, W.: Neuronal representations, assemblies and temporal coherence. In T.P.Hicks et all: Progress in Brain Research. vol.95, Elsevier, 1993, Chapter 37, pp. 461-474




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2017: Because of retirement the former page
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Find the new table of contents at http://www.gheinz.de/

Mail to Andrew Packard: andrew@packards.de
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File created Nov. 24, 1998 gh/ap.
First revision in May 2005: text corrections.
Second revision in November 2020: Redesign; mpg-files converted to mp4; sources AP06, AP11, Hz01 added; Fig.13 added, small textual corrections and adds, new layout. Januar 2022: Stylesheet included.